Developmental competence of parthenogenetic mouse and human embryos after chemical or electrical activation
نویسندگان
چکیده
منابع مشابه
Efficient Method For Combined Electrical-Chemical Parthenogenetic Activation of Bovine Oocytes
Purpose: Parthenogenetic activation is among the crucial steps determining successful development of mammalian cloned embryos. This study, therefore, was conducted to evaluate the efficiency of a novel combined electrical-chemical artificial activation method for bovine cloning. Materials and Methods: In vitro matured bovine oocytes than were initially exposed to electrical pulse, used for cel...
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Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...
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Background: Artificial oocyte activation (AOA) has important implications in assisted reproductive technique (ART) with particular importance in somatic cell nuclear transfer (SCNT). Parthenogenesis activation using zona- free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. Materials and Methods: To establis...
متن کاملThe developmental fate of androgenetic, parthenogenetic, and gynogenetic cells m chimeric gastnflating mouse embryos
Both a maternal and a paternal genomic contribution are necessary for completion of embryonic development in the mouse. Parthenogenetic embryos, with only a maternally inherited genome, and androgenetic embryos, with only a paternally inherited genome, fail to develop to term, and these two types of isoparental embryos fail in development in characteristic ways. In this paper we describe the co...
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In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 μg/ml) of vitamin C supplemented in in vitro maturation (IVM) and culture (IVC) media were ...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2010
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2010.07.001